Application of Supercritical CO2 Extraction Technology in the Extraction of Terpenoids as Effective Components of Traditional Chinese Medicine
Comparison of two methods for extracting paclitaxel
Paclitaxel discovered in the 1960s, is a diterpenoid with anti-cancer activity derived from the bark of Taxus brevifolia. It has good therapeutic effects on ovarian cancer and breast cancer, and has been a hot spot in the past 20 years. One of the most anti-cancer drugs.
SFCE VS Ethanol extraction
Used supercritical CO2 fluid to extract and separate paclitaxel from yew branches and leaves.
After experiments, it was determined that the coarse powder of yew branches and leaves with a particle size of 0.25～0.45 mm was extracted at a pressure of 27.6 MPa, a temperature of 31 ℃ and methanol as an entrainer. The extraction was carried out under the conditions of the absorption liquid, and the paclitaxel was completely extracted within 2 h, the extraction rate was 96.5%, and the mass fraction of paclitaxel in the extract could reach 1%.
Compared with the traditional ethanol three-time extraction method, supercritical CO2 fluid extraction has a simple technical process, few steps, short time-consuming, high extraction rate, and no residual solvent residue, making it a better extraction method.
What is the process for supercritical CO2 extraction of pandanus leaves?
Used supercritical CO2 to extract the effective ingredients in Pandanus leaves. The extraction conditions were determined by experiments as follows: pressure 45 MPa, temperature 50 ℃, extraction time 3 h, and 3% absolute ethanol as the entrainer. The extraction rate highest.
Analyzed by GC-MS technology, the extract mainly contains terpenoids such as squalene, which have certain health effects.
How to extract the terpenoids in thyme with supercritical CO2？
Used supercritical CO2 extraction technology to separate the effective components of terpenoids in thyme.
In the experiment, investigated the influence of pressure, temperature and entrainer on the extraction effect, and finally determined the optimal extraction conditions as pressure 15 MPa, temperature 50 ℃, 3 % Ethanol is the entrainer, and 97% monocyclic terpenoid extract can be obtained under this condition.