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3 kinds of traditional Chinese medicine CO2 extraction process

Optimizing the supercritical fluid extraction process

When optimizing the process of applying supercritical fluid extraction technology to extract volatile oil, the commonly used optimization method is the orthogonal test method.

The extraction rate of volatile oil is usually used as an indicator to investigate factors such as extraction pressure, extraction temperature, pulverization degree, and extraction time.

supercritical CO2 fluid extraction Perilla leaf volatile oil

The supercritical CO2 fluid extraction volatile oil from perilla leaves was studied by single factor experiment and orthogonal experiment. The effects of extraction pressure, extraction temperature, extraction time and CO2 flow rate on the yield of volatile oil were investigated, and the volatile oil was analyzed by gas chromatography-mass spectrometry. chemical composition.


The effect of supercritical CO2 fluid extraction on the yield of volatile oil from perilla leaves is as follows:

CO2 flow> extraction temperature> extraction time> extraction pressure;

What is the best CO2 extraction method for volatile oil from perilla leaves?

The pressure is 20 MPa, the temperature is 35 ℃, the flow rate is 10 kg/h, and the time is 150 min. Under this condition, the yield of volatile oil reached 3.2%, and 16 compounds were identified, among which the content of parsyl ether was the highest, reaching 42.43%.

Gan Zagari and Bi Bilin

Supercritical CO2 fluid extraction was used to study the chemical composition of the essential oils of Tibetan medicine Gan Zagari and Bi Bilin.

Gan Zagari and Bi Bilin is one of the simplest compound prescriptions of Tibetan medicine, consisting of Gan Zagari and Bi Bi Lin.


The yield of essential oil was 5.9%; 48 chromatographic peaks were separated by gas chromatography-mass spectrometry, and 43 components were identified.

Main ingredients

Eucalyptus oil diene-5,11(13)-lactone-8,12, piperine, α-lupulene, elemene, trans caryophyllene, etc., of which Eucalyptus oil diene-5,11(13 )-The sum of the relative content of lactone-8,12 and piperine accounts for 39.40% of the essential oil content.

Similar to the single herbs Ganzhagari and Bibilin, the main medicinal substances contained in Ganbier Decoction are piperine, trans-caryophyllene and elemitol.

However, eucalyptus oil diene 5,11(13)-lactone-8,12, hydrogenated cinnamic acid and other components detected in Ganbi Ertang are used in the essential oils of single medicine Ganzhagari and Bibilin. Not found in the ingredients.

Tongjingbao Granules

Using the contents of ligustilide, cinnamic aldehyde, costanolide and dehydrocostuslactone as chemical indicators, the supercritical CO2 fluid extraction technology of the volatile oil of angelica, cinnamon and woody in Tongjingbao granules was optimized, and A mouse model of dysmenorrhea and a rat model of uterine spasm were used to compare the efficacy of the preparation before and after the process improvement.


The method of extracting volatile oil from angelica alone and combining cinnamon and woody is better with supercritical CO2 fluid extraction technology. After the process is improved, the antispasmodic effect of the preparation is significantly enhanced compared with the original preparation.

Photo of Supercritical CO2 Chinese Medicine Extraction Equipment

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