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Supercritical CO2 Fluid Extraction Of Flavonoids And Their Glycosides

This article provides methods and process parameters for supercritical carbon dioxide extraction of flavonoids from four herbs.

Four herbs: Phellinus linteus, Ginkgo biloba, Codonopsis, Polygonum cuspidatum.

Flavonoids

Flavonoids generally refer to natural products with a basic structure of C6-C3-C6, most of which are chromone derivatives.

They are widely found in vegetables, fruits, pastures and medicinal plants. They are the natural selection process of plants in the long-term Some secondary metabolites produced.

Phellinus linteus

Wang Xinyu et al used supercritical CO2 to extract the flavonoids in the process of extracting the flavonoids.

Supercritical CO2 Extraction Of Phellinus Linteus
Supercritical CO2 Extraction Of Phellinus Linteus

The extraction rate of flavonoids was used as an indicator, and the effect of pressure, temperature, CO2 flow rate and the amount of entrainer on supercritical extraction was investigated by single factor experiment and orthogonal design method.

Influence and compared with ultrasonic extraction method.

The optimal extraction conditions (calculated based on 300 g sample) are pressure 35 MPa, entrainer dosage 200 mL, CO2 flow rate 10 L/h and temperature 45 ℃. Under these conditions, the mass fraction of supercritical extract flavonoids is ultrasonic the extract is 67 times more efficient than traditional extraction methods and is suitable for large-scale extraction of Phellinus linteus.

Extracting ginkgo biloba flavonoids

In the study of extracting ginkgo biloba flavonoids by supercritical CO2 fluid extraction method, Zhao Qijun and others investigated the extraction pressure, temperature, time and the volume fraction of the entrainer, and found that the extraction pressure is the most important factor affecting the extraction effect, and the temperature is the second factor, and the volume fraction of entrainer and extraction time have relatively little influence on the experimental results.

Supercritical CO2 Extraction Of Ginkgo Biloba Flavonoids
Supercritical CO2 Extraction Of Ginkgo Biloba Flavonoids

The optimal extraction conditions are finally determined as follows: extraction pressure 35 MPa, temperature 50 ℃, time 1.5 h, volume fraction of entrainer 90%.

Compared with the traditional method, the SFE method is safer without solvent residues, and can be reused and more economical.

Extract the active constituents of Codonopsis codonopsis

Liu Tongju and others used the supercritical fluid extraction method to extract the main active components of Codonopsis pilosula, and used the central experimental design combined with the response surface method to study the influence of factors such as pressure, temperature, extraction time, entrainer dosage and other factors.

Supercritical CO2 Extraction Of Codonopsis
Supercritical CO2 Extraction Of Codonopsis

The determination of the amount of entrainer is a key factor in the whole process.

Finally, it is concluded that when the volume flow rate of dynamic entrainer ethanol is 1 mL/min and the atmospheric CO2 flow rate is 2 L/min, 30 MPa, and 60 ℃, extraction is performed for 100 min. The glycoside yield was 0.078 6 mg/g, which was better than the traditional ultrasonic extraction method.

Extraction Japanese knotweed

Benova et al used the supercritical CO2 fluid extraction method to extract the effective components of resveratrol glycosides and emodin in Japanese knotweed.

Supercritical CO2 Extraction Of Japanese Knotweed
Supercritical CO2 Extraction Of Japanese Knotweed

The optimal conditions were determined by experiments as follows: pressure 40 MPa, temperature 100 ℃, extraction time 45 min, entrainer acetonitrile, and The traditional Soxhlet extraction method is compared, and it is found that the time has been shortened by nearly 5/7, and the yield has also been significantly improved.

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