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The method and conclusion of supercritical CO2 extraction of Antarctic krill oil

Antarctic Krill Oil

Antarctic krill oil is derived from pure wild Antarctic krill and extracted by ultra-low temperature extraction. It is a natural health food gifted by nature to humans, and contains effects that other foods cannot replace.


It is clinically proven that krill oil has super-strong hypolipidemic, lowering blood sugar, lowering blood pressure, crossing the blood-brain barrier nutrition and activating brain cells and nerves, which can prevent and treat cerebrovascular diseases; at the same time, it can prevent and treat coronary atherosclerosis. Sclerosis, coronary heart disease, prevention of cardiovascular diseases such as myocardial infarction, stent, bypass and secondary recurrence;

Of course, Antarctic krill oil can also have multiple functions such as antibacterial and anti-inflammatory, preventing the occurrence and development of cancer, delaying aging, and regulating endocrine. Currently, 300 million people in the world consume Antarctic krill oil.


Antarctic krill oil contains Omega-3 essential fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA).

Omega-3 essential fatty acids have many health-promoting benefits, including cardiovascular, nerve, bone and joint, vision, skin health, etc.

Unsaturated fatty acid

Its unsaturated fatty acid is currently the only molecular structure in nature that combines omega-3 (EPA, DHA) and a variety of super antioxidants (phospholipid-type Astaxanthin) in the form of phospholipids. It is also rich in vitamin E, vitamin A, astaxanthin, phospholipids, flavonoids and powerful antioxidants.

Method of supercritical CO2 extraction of Antarctic krill oil

Antarctic Krill Oil Supercritical CO2 extraction

antarctic krill oil supercritical CO2 extraction equipment
antarctic krill oil supercritical CO2 extraction equipment

The supercritical CO2 extraction pressure (300bar, 35bar and 40°C) and temperature (20°C, 25°C and 300bar) are used as extraction factors to optimize. The extracted Antarctic krill residue is extracted with ethanol and n-hexane to extract Antarctic phosphorus Shrimp lecithin.

Oil recovery rate

The results show that when the supercritical CO2 extraction method does not add a co-solvent, the extraction condition is 35°C, and the extraction is performed at 30 MPa for 1 hour, the oil recovery rate is the highest, reaching 55.47%;

The addition of 10% ethanol of shrimp powder mass as a co-solvent can increase the oil recovery rate, and the oil recovery rate will not increase with its addition amount.

The Antarctic krill residue powder after supercritical CO2 extraction was re-extracted with ethanol and n-hexane, and the oil extraction rates were 7.62% and 3.26%, respectively.

Phospholipid content

The Antarctic krill oil directly extracted by supercritical CO2 extraction contains 88.18% triglycerides, 2.34% free fatty acids and 5.82% cholesterol. The phospholipid content in the shrimp oil extracted with ethanol as a co-solvent is only 2.91%.

Shrimp oil extracted without co-solvent contained 31.08% PUFA, 35.69% MUFA and 33.24% SFA, and the values ​​after extraction with entrainer were 17.40%, 33.96% and 48.64%, respectively.

Acid value

The acid value of the shrimp oil extracted without cosolvent and with cosolvent was 6.24mgKOH/g and 12.92mgKOH/g, respectively. In the oils extracted with ethanol and n-hexane, polar oils account for 100% of the total lipids.

PUFA content

At the same time, the PUFA content in the shrimp oil extracted by ethanol and n-hexane was 33.18% and 35.95%, respectively. The shrimp oil extracted by this method has a higher acid value.


The above results indicate that the supercritical CO2 extraction method cannot effectively extract phospholipids from krill, but it can remove the neutral oils in shrimp oil. High-purity Antarctic krill phospholipids can be obtained from the Antarctic krill residue powder extracted by supercritical CO2 plus co-solvent method.

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