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Development Of Supercritical Extraction Technology For Papaya Seeds

Recently, BIT Laboratory has completed the research and development support task for a project of a university in the south: research on supercritical extraction technology of papaya seed oil.

What Is Supercritical CO2 Extraction?

Supercritical carbon dioxide extraction is a commonly efficient separation method used method to separate various active ingredients from the plant due to it producing a pure.

Supercritical co2 solvent extraction is one of the gentlest, most flexible, dynamic, CO2 Extraction allows you to create a pure, clean, quality oil that is safe to produce with little-to-no post-processing. These are the products extracted at ambient temperatures and at high pressures to avoid loss of aroma and degradation of actives, also, since supercritical fluid extraction is a green process and nature friendly techniques, it can be used to the extraction of spices, herbs and flowers using food grade CO2.

Principle Of Supercritical CO2 Extraction Process

By changing the temperature and pressure as well as flow rate, certain molecules will bond to CO2, allowing them to be separated from the plant.
In the supercritical state, supercritical carbon dioxide is brought into contact with the substance to be separated, so that it selectively extracts the components with different polarity size, boiling point height and molecular weight size in turn. Of course, the extracts obtained in each pressure range cannot be single, but the conditions can be controlled to get the best proportion of mixed components, and then the supercritical fluid is turned into ordinary gas by means of pressure reduction and temperature increase, and the extracted substances are completely or basically precipitated out, so as to achieve the purpose of separation and purification, so the supercritical fluid carbon dioxide extraction process is a combination of extraction and separation. Supercritical carbon dioxide extraction is a commonly used method to separate various components from the plant due to it producing a pure, clean, and safe product.

7 Featuress Of The Solubility Of Supercritical CO2 Fluids

In the supercritical state, the solubility of CO2 for different solutes varies greatly, which is closely related to the polarity, boiling point and molecular weight of the solute, and generally has the following features:

  1. Lipophilic, low boiling point components can be extracted below 104KPa (about 1 atm), such as volatile oils, hydrocarbons, esters, ethers, and epoxides.
  2. Aroma components from natural plants and fruits, such as eucalyptus brain, muscimol, low boiling point esters from hops, etc.
  3. The more polar groups ( such as -OH, -COOH, etc.) of a compound, the more difficult it is to extract.
  4. The extraction pressure of strongly polar substances: sugar and amino acids should be above 4×104KPa.
  5. The larger the molecular weight of the compound, the more difficult it is to extract.
  6. Components with molecular weight in the range of 200-400 are easy to extract, and some low molecular weight, volatile components can even be extracted directly with CO2 liquid.
  7. High molecular weight substances (such as proteins, gums, waxes, etc.) are very difficult to extract with CO2.

Works Cited: CO2 Extraction Process: Basics, 5 Advantages, 7 Features, 4 Applications

Supercritical CO2 Extraction Equipment for Papaya Seed Oil


Papaya is also known as papaya, pumpkin, milkmelon, tree winter melon and longevity fruit.

It is widely planted in Guangdong, Guangxi, Hainan, Yunnan and Fujian provinces. Papaya is not only a fresh fruit, but also a large number of processed beverages, enzymes, juices and jams and other products. Papaya seed is the waste material of papaya pulp processing, accounting for about 7% of the weight of papaya.

Papaya seed oil

Supercritical Extraction Technology For Papaya Seeds
Supercritical Extraction Technology For Papaya Seeds

Papaya seed oil is a kind of monounsaturated fatty acid oil mainly composed of oleic acid, which is very similar to olive oil, but has obvious differences with other vegetable oils (such as peanut oil, rapeseed oil, etc.). Oleic acid plays a special role in lipid metabolism. It can not only reduce harmful cholesterol (LDL), but also maintain the level of beneficial cholesterol (HDL), thus slowing down atherosclerosis and effectively prevent cardiovascular diseases such as coronary heart disease. High oleic acid content is conducive to prolonging the shelf life of oils and products. Nutrition makes oleic acid a “safe fatty acid”.

The oleic acid content of papaya seed oil is as high as 68.42%. Therefore, the development of papaya seed oil as a food or health product oil will be very beneficial to the human body.

Compared with traditional extraction and separation technology, supercritical extraction has unique advantages such as low temperature, effective retention of biological activity, good product quality, etc.

Therefore, the application of supercritical extraction technology in papaya seed extraction, if successful, will have rich product development possibilities and market application prospects.

Extraction experiment

The goal and technical difficulty of the research and development support provided by BIT Laboratory for a project team of Southern University is to find a suitable supercritical extraction process route and operation parameters, so as to achieve a higher extraction rate while taking into account the quality of the extracted product oil, so as to minimize the number of the whole extraction process of papaya seeds, save production time, and as far as possible. Reduce the equipment investment and production cost of enterprises in the future.

Through many days of in-depth research and experimental verification, BIT Lab successfully found the determinants and changing rules to ensure the quality and improve the yield of papaya seeds as much as possible, and successfully completed the technical development work. Samples and research and development reports have been delivered to the project team of the University today for the follow-up research and development work.


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