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Supercritical CO2 extraction of peony seed oil

Peony seed oil

Peony seed oil contains linolenic acid, linoleic acid, DHA, paeonol, peony saponin, peony polysaccharide, fucosterol, squalene and other more than 100 nutritional and physiological active substances. The main effects are antioxidant, anti-tumor, In terms of antibacterial and anti-inflammatory, lowering blood fat, lowering blood sugar, and protecting liver damage, it is hailed as “the best oil in the world” by nutritionists.

Supercritical CO2 extraction of peony seed oil
Supercritical CO2 extraction of peony seed oil

Toxicological studies have shown that peony seed oil has no acute toxicity, genetic toxicity and subacute toxicity, and has high edible safety.

Supercritical CO2 extraction

Supercritical CO2 extraction has good critical conditions, no solvent residues, low pollution to the ecological environment, selective extraction, good solubility in oils, fewer process steps, simple separation, short time-consuming, and high extraction rate. During the operation, the temperature is relatively low, the physiologically active substances are best preserved, the obtained oil products contain no impurities, the oil has good color and high purity, and the physical and chemical properties of the oil obtained are better than those obtained by traditional agent extraction. The advantages are known as the green technology, so It is widely used in functional vegetable seed oil extraction.

The supercritical CO2 extraction of peony seed oil is light in color, transparent and clear, with high refractive index, relative density, unsaturated fatty acid content, low saponification value and peroxide value content, and its quality is better than Soxhlet extraction and chemical solvent extraction , Squeezing method and the oil obtained from the water substitution method.

Principle of supercritical CO2 extraction of peony seed oil

Features

Supercritical CO2 extraction technology is an “environmentally friendly”, high-efficiency, high-yield integrated separation technology. It has strong solubility for fat-soluble components and high safety. It is suitable for the extraction of fat-soluble, high-boiling, and heat-sensitive substances. .

Basic principle

  • At present, the extraction of peony seed oil widely uses supercritical CO2 extraction technology.
  • The basic principle is that the CO2 fluid contacts the substance to be extracted in the supercritical state, and the mass transfer effect of the CO2 solvent above the critical temperature is close to or even better than that of the liquid. Quickly penetrate into the voids of the material, which is beneficial to the extraction of the material to be extracted;

  • Increase the pressure to increase the solubility of the substance to be extracted, so that it can be dissolved in the CO2 fluid, and the required effective components of polarity, boiling point, and molecular mass are selectively extracted in sequence, and then with the help of decompression and heating The method reduces the solubility of the substance to be extracted and precipitates, so as to achieve the purpose of extraction and separation.

Technological process of supercritical CO2 extraction of peony seed oil

The supercritical CO2 extraction process is simple and mainly consists of two parts: supercritical CO2 extraction of solutes and separation of substances to be extracted from supercritical CO2.

Peony seed crush

Choose fresh, full, and pest-free peony seeds, wash them manually or mechanically to remove hulls and remove impurities (hull content <8.00%, impurity content <0.04%), and dry in an oven at 45-60°C for 48h ( Vacuum drying for 2h) to a constant quality (3.5% moisture content), crush and dry peony seeds to a certain size with a grinder, and sieve the peony seed powder particles through a 20-100 mesh sieve to prevent overheating of the pulverizer from causing damage to the peony seed oil Oxidation.

Set extraction parameters

Set a certain extraction pressure, temperature, and time. Set the pressure of separation vessel I to 10MPa and temperature of 35°C during supercritical extraction; the pressure of separation vessel II is 5~6MPa, and the temperature is 26~28°C. Weigh the treated peony seed powder (The extraction feed volume is 25%-40% of the volume of the extraction kettle) Put it into the extraction kettle, turn on the CO2 cylinder, open the refrigeration valve of the cold cycle, and pressurize the system with a high-pressure pump.

When the refrigeration equipment stops working, open the high-pressure pump and open the corresponding valve to maintain the path state; adjust the flow of carbon dioxide (CO2 flow 25-30L/h) through the flowmeter, and maintain the cycle for an appropriate time (the extraction time is determined by the oil content of the seed, The oil content of the raw material seeds is greater than 15%, and the general extraction time is 2 to 3 hours.

Ethyl acetate extraction

After the extraction is completed, the discharge valve of the separation kettle is opened, and the light yellow translucent oily extract in the separation kettle is collected and extracted with ethyl acetate. After the ethyl acetate was evaporated, it was dried with anhydrous sodium sulfate overnight to obtain a pale yellow oil.

Oil yield

The oil yield or extraction rate of peony seed oil extracted by supercritical CO2 is significantly different, and the specific performance is between 23.85%-30.7% or 3.87%-98.55%. Further analysis found that the oil content of peony seeds in different peony raw materials origins or varieties is not affected.

It is generally believed that as the altitude increases, the light intensity increases, which is beneficial to increase the oil content of peony seeds. The oil content of Fengdan peony seeds is generally between 24.12% and 33%, and the oil content of Paeonia edulis seeds is 37.8%. Beyond influence

The main factors affecting the oil yield or extraction rate of peony seeds in the supercritical CO2 extraction process are the particle size of the material, the CO2 flow rate, the extraction pressure, the extraction temperature, and the extraction time.

The sequence of factors affecting the oil yield of peony seeds is: extraction pressure>CO2 flow rate>extraction temperature>extraction time, and other factors such as the different injection volume of peony seeds and water content of peony seeds.

Oil production

The oil yield or extraction rate of peony seed oil extracted by supercritical CO2 is significantly different, and the specific performance is between 23.85%-30.7% or 3.87%-98.55%.
Further analysis found that the oil content of peony seeds in different peony raw materials production areas or varieties is different;
It is generally believed that as the altitude increases, the light intensity increases, which is beneficial to increase the oil content of peony seeds;
The oil content of Fengdan peony seeds is generally between 24.12% and 33%, and the oil content of the seeds of Paeonia vulgaris is 37.8%.

Factors affecting the extraction rate of supercritical CO2

The main factors affecting the oil yield or extraction rate of peony seeds in the supercritical CO2 extraction process are the particle size of the material, the CO2 flow rate, the extraction pressure, the extraction temperature, and the extraction time.

The sequence of factors affecting the oil yield of peony seeds is: extraction pressure>CO2 flow rate>extraction temperature>extraction time, and other factors such as the different injection volume of peony seeds and water content of peony seeds.

The sequence of factors affecting the oil yield of peony seeds is: extraction pressure>CO2 flow>extraction temperature>extraction time.

Selection of technological conditions for supercritical CO2 extraction of peony seed oil

The process parameters of supercritical CO2 extraction of peony seed oil are as follows:

peony seed oil supercritical CO2 extraction equipment
peony seed oil supercritical CO2 extraction equipment

Material vacuum drying 1.5~2h, process sieve crushing particle size 40~60 mesh, 1L extraction kettle feed volume 250~300g, CO2 flow rate 20~30L/h, extraction pressure 30~35MPa, extraction temperature 35~55℃, analysis pressure 9 ~10MPa, analysis temperature 50~60℃, time 1~2.5h, the extraction rate of peony seed oil can reach more than 23%, and the effective extraction rate can reach more than 93%.

Processing quality of peony seed oil extracted by supercritical CO2

Acid value, iodine value, saponification value, peroxide value, appearance properties and stability are important indicators to measure the quality of oils. The ideal oil extraction technology and oil extraction process should not only have high oil yield, fatty acid composition, and VE content. , Should also make the oil have good color and smell.

Because peony seed oil is rich in unsaturated fatty acids, supercritical CO2 extraction technology will reduce the oxidation rate of peony seed oil in the extraction process to a certain extent, and its extraction rate of peony seed oil is higher than traditional extraction methods.

The appearance of peony seed oil extracted by supercritical CO2

  • The use of supercritical CO2 to extract peony seed oil can protect the physiological activity of active substances. It is light yellow (Y10.0, R1.0), has a unique peony aroma, and is rich in essential unsaturated fatty acids. The prepared peony seed oil contains Less phosphorus, low peroxidation rate;
  • The extracted oil is clear and transparent, light red to yellow transparent liquid;
  • When comparing the quality and activity of Dian peony seed oil and Fengdan peony seed oil, it was found from the appearance that Dian peony seed oil was brown and transparent oily liquid; Fengdan peony seed oil was slightly lighter in color and was light yellow, transparent oily liquid.

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